Herein we show that an innovative maskless x-ray nanopatterning approach allows writing electrical gimmick with nm feature size . In especial we construct a Josephson device on a Bi2Sr2CaCu2O8+δ ( Bi-2212 ) superconducting oxide micro-crystal by cast two individual lines of only 50 nm in breadth exploitation a 17.4 keV synchrotron nano-beam . A accurate control of the fabrication process was achieved by monitoring in situ the version of the twist electrical impedance during X-ray actinotherapy , thus finely tuning the actinotherapy time to get the substantial into a non-superconducting land only in the irradiated neighborhood , without importantly perturbing the lechatelierite structure . Time-dependent finite element model simulations show that a possible microscopical origin of this effect can be related to the instant temperature increase induced by the vivid synchrotron picosecond X-radiation pulses . These ensue examine that a conceptually new model method for oxide electric devices , base on the local vary of electrical belongings , is really potential with possible vantage in condition of heat licentiousness , chemical pollution , miniaturization and high facet A Glass-Ultra-Thin PDMS Film-Glass Microfluidic Device for Digital PCR lotion Based on Flexible Mold Peel-Off Process . DEHYDROMUCIC ACID ( MFD ) with a glass−PDMS−glass ( G-P-G ) construction is of interest for a wide range of applications . notwithstanding , G-P-G MFD fabrication with an ultra-thin PDMS film ( especially heaviness less than 200 μm ) is stillness a big challenge because the ultra-thin PDMS film is easy twist , curled , and damaged during demolding and transferring . 2, 5-Furandicarboxylic acid aimed to report a thickness-controllable and low-priced fictionalization action of the G-P-G MFD with an ultra-thin PDMS film based on a flexible mold peel-off process . A patterned photoresist stratum was deposited on a polythene terephthalate ( PET ) film to invent a flexible mold that could be demolded quietly to achieve a rigid structure of the glass−PDMS film . The thickness of ultra-thin patterned PDMS could reach less than 50 μm without price to the PDMS film . The MFD showcased the first-class property of irrigate desiccation forbiddance ( pee loss < 10 % ) during PCR thermal bicycle because of the ultra-thin PDMS film . Its low-cost fabrication process and fantabulous water evaporation prohibition present super high candidate for digital PCR application . A microfluidic chopine for chemic arousal and real time analysis of catecholamine secernment from neuroendocrine cells.Release of neurotransmitters and hormones by calcium-regulated exocytosis is a fundamental cellular work that is interrupt in a salmagundi of psychiatric , neurological , and endocrine unhinge . As such , thither is meaning interest in targeting neurosecretion for drug and therapeutical maturation , efforts that will be aided by novel analytic prick and devices that provide mechanistic penetration conjugate with increase observational throughput . Here , we report a simple , cheap , reclaimable , microfluidic device designed to analyze catecholamine secretion from pocket-sized universe of adrenal chromaffin cadre in real time , an crucial neuroendocrine element of the sympathetic queasy scheme and versatile neurosecretory model . The device is fabricated by replication molding of polydimethylsiloxane ( PDMS ) use patterned photoresist on Si wafer as the original . Microfluidic recess channels lead to an raiment of U-shaped `` cell traps '' , each capable of immobilizing bingle or small groups of chromaffin cells . The stern of the gimmick is a glaze skid with patterned thin film Pt electrodes used for electrochemical spotting of catecholamines in real time . We demonstrate authentic load of the device with belittled universe of chromaffin cells , and perfusion/repetitive stimulation with physiologically relevant secretagogues ( carbachol , PACAP , KCl ) victimization the microfluidic net . evoke catecholamine secretion was reproducible over multiple rounds of arousal , and place as expect to different tightness of secretagogue or removal of extracellular Ca . overall , we show this microfluidic device can be used to enforce composite stimulation epitome and analyze the amount and dynamics of catecholamine secretion from little populations of neuroendocrine cadre in real Fabrication of focalise open plasmon sonorousness sensors with scalable polyvinyltetrazole/copper cluster crossbreed ring-array for Cu ( II ) detection.of Science and Technology , 43 , Sec . 4 , Keelung Road , Taipeh , 106 , Taiwan , ROC .
DEHYDROMUCIC ACID|2, 5-Furandicarboxylic acid
